Last edited by Kajijas
Friday, July 31, 2020 | History

4 edition of Ion-Sensitive Intracellular Microelectrodes found in the catalog.

Ion-Sensitive Intracellular Microelectrodes

Roger Christopher Thomas

Ion-Sensitive Intracellular Microelectrodes

How to Make and Use Them (Biological techniques series)

by Roger Christopher Thomas

  • 175 Want to read
  • 20 Currently reading

Published by Academic Pr .
Written in English

    Subjects:
  • Electrodes, Ion selective,
  • Microelectrodes,
  • Technique,
  • Cytology

  • The Physical Object
    FormatHardcover
    Number of Pages123
    ID Numbers
    Open LibraryOL9283874M
    ISBN 100126887500
    ISBN 109780126887501

    The microelectrode technique is today the most widely used method in electrophy- siology. Microelectrodes offer a unique approach to measurements of electrical pa- rameters and ion activities of single cells. Several important breakthroughs in trans- port physiology have arisen from microelectrode studies. Undoubtedly, there is a progressively wide-spread use of conventional and ion-selective. Thomas RC, Moody WJ () Ion-sensitive microelectrodes for intracellular use. Trends Biochem Sci –87 Google Scholar Thomas RC, Oehme M, Simon W () Lithium accumulation by snail neurones measured by a new Li + -sensitive by:

    Ion-sensitive intracellular microelectrodes: how to make and use them / R. C. Thomas; Ion-selective microelectrodes, edited by Herbert J. Berman and Normand C. Hebert; The Application of ion-selective microelectrodes / editor, Thomas Zeuthen. Ion-sensitive intracellular microelectrodes: how to make and use them / R. C. Thomas Ion-selective microelectrodes, edited by Herbert J. Berman and Normand C. Hebert The Application of ion-selective microelectrodes / editor, Thomas Zeuthen.

    Procedures for manufacturing double-barrelled ion-sensitive microelectrodes employing liquid sensors. Journal of Biochemical and Biophysical Methods , 13 (1), DOI: /X(86) M. Desilets, C. M. by: Fozzard H. A., Chapman R. A., and Friedlander I. R. () Measurements of intracellular calcium ion activity with neutral exchanger ion sensitive microelectrodes. Cell Calcium 6, .


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Ion-Sensitive Intracellular Microelectrodes by Roger Christopher Thomas Download PDF EPUB FB2

This must have many consequences to my thoughts on experiments with microelectrodes. In this book, my concern is fo­ cussing on the description of an intracellular method that should lead to reliable in­ formation on cellular parameters.

The methodical basis for any meaningful applica­ tion is Cited by: at a cellular level. Ion-sensitive microelectrodes provide a means of directly assessing the extracellular or intracellular activity of an ion and for making prolonged measurements of these.

It is the activity of an ion which is important because it, rather than the total or free concentration, determines, for example, the membrane potential,File Size: KB. The Symposium on "Ion Selective Microelectrodes and Their Use in Excitable Tissues" was held in Prague from JulyIt was organized by the Institute of Physiology of the Czechoslovak Academy of Sciences as a satellite symposium of the XXVIII Interna­ tional Congress of Physiological Sciences in : Paperback.

Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (K), or click on a page image below to browse page by page. Ion-sensitive microelectrodes provide a means of directly assessing the extracellular or intracellular activity of an ion and for making prolonged measurements of these.

It is the activity of an ion which is important because it, rather than the total or free concentration, determines, for example, the membrane potential, equilibrium potentials, or the thermodynamic conditions for ion transport mechanisms.

Microelectrode Methods for Intracellular Recording (Biological Techniques Series): Economics Books @ 5/5(1). For H + injection microelectrodes were pulled from quartz tubing filled with 1 M HCl and left with tips intact.

Intracellular CaSMs were made from quartz glass and pHSMs from borosilicate glass [30, 31]. For surface pH, both liquid ion sensor and Hinke-style glass pH microelectrodes [32] were used.

The pH-sensitive microelectrodes responded with 55 to 60 mV (at 35 °C) to a unit change of the pH in the bathing solution, the response being more than 90% complete within 30 to s.

The response times of the ion-sensitive microelectrodes used in our experiments were much faster than the changes in the intracellular ion activities : Joachim W.

Deitmer, David Ellis. Performance of quartz ion-selective microelec- trodes Apart from their greater mechanical strength, which means that they penetrate tough connective tissue, do not become blunt, and can be wiped quite vigorously between the fingers, quartz ion- 63 selective microelectrodes for intracellular measure- ments, made as described, appear to function longer than those made of Pyrex by the method of Cited by: the use of ion-sensitive electrodes or ionophoresis of drugs.

Making microelectrodes One definition of a microelectrode (ME) might be: ‘an electrode constructed with a tip having the dimensions of the order of a micrometre (1 µm)’. Usually this means a glass micropipette of the type pioneered by Ling & Gerard (), which is filledFile Size: KB.

Emerging techniques: Ion-sensitive microelectrodes for intracellular use. TIBS 5, (). Reversal of the pHi-regulating system in a snail neuron. In: Current Topics in Membranes and Transport, 13, pp.ed. E.L. Boulpaep. Regulation of intracellular Na+, K+ and H+ concentrations in snail neurons.

Construction of these ion-sensitive microelectrodes for intracellular use remains an art, since all of the alternative fabrication procedures have not yet been explored. TF~is article will review the construction and use of these Ca +-sensitive microelectrodes according to the methods currently used in our labs, Cited by:   Part of the Neuromethods book series (NM, volume 43) Ca 2+ -sensitive microelectrodes are time-consuming to make and require large robust cells.

But, they do not add to buffering and do not require expensive equipment. I describe how to make and use the electrodes and briefly consider the leakage by: 2.

This website uses cookies to ensure you get the best experience on our website. Learn moreAuthor: Jeffrey L. Noebels. The Yale Journal of Biology and Medicine [01 Sep52(5)]Author: JL Noebels. Theory, Potential and Existing State of Development.- 1. Perspective: Ion-Selective Microelectrodes: Their Potential in the Study of Living Matter In Vivo.- 2.

Ion-Selective Electrode Response in Biologic Fluids.- 3. Glass Microelectrodes for pH.- 4. Some Problems with an Intracellular PO2 Electrode.- 5. Some Problems with the Antimony. A recipe for making double-barrelled ion-sensitive microelectrodes is given.

Introduction The activities of a number of different ionic species can now be measured in biological tissues with ion-sensitive microelectrodes of the type in which a column of liquid sensor is lodged in the open tip of a glass micropipette (see e.g.

Ammann et al., ).Cited by: The measurement of intracellular ionic activity is crucial to a quantitative understanding of neuronal function. Intracellular ion-sensitive microelectrodes (ISMs) can now be routinely made which are capable of determining the ionic activities of Na +, K +, Cl −, H + and Ca 2+ and of monitoring their changes even in small neurones.

This article outlines some of the theoretical and practical Cited by: intracellular ‘sharp’ microelectrode recording; intracellular microelectrodes, and advantages; impalement, and cell stress; sharp intracellular microelectrodes; electrode capacitance; microelectrode amplifiers, current through microelectrode; amplitude of changes in membrane, neuronal action potential.

Abstract. Ca 2+-sensitive microelectrodes generate a voltage proportional to the logarithm of the calcium activity (pCa) at their principle they are simple to understand and calibrate, but difficult to make and use intracellularly.

Their main advantages over other methods of measuring pCa i (such as fluorescent dyes and luminescent proteins) are that they measure pCa at one point and Cited by: 4. Intracellular pH (pH i) has been studied extensively for over a century using a wide range of techniques.

These techniques have been subject to constant improvements to the extent that useful measurements can now be made in even the smallest of cells. This chapter outlines the development of ion-sensitive microelectrodes and dyes and highlights.The resting pH of the rat lens was determined using H +-sensitive liquid membrane microelectrodes and found to be 689 when measured in a perifusing solution of pH 7 The pH of the rat vitreous humour was also measured and was found to be 7Cited by: Ion-sensitive microelectrodes (ISEs) have been used to measure intracellular [Mg2+] ([Mg2+]i) in cardiac muscle, although most measurements have tended to overestimate the value due to the poor selectivity of the Mg2+ ionophore in the sarcoplasm and to inaccurate collation of individual ISE measurements.

This paper highlights the correct method Cited by: 8.